The Battle of Ayubale results in the destruction of most of the Spanish missions in Florida.

The Apalachee massacre was a series of raids by English colonists from the Province of Carolina and their Indian allies against a largely peaceful population of Apalachee Indians in northern Spanish Florida that took place in 1704, during Queen Anne's War. Against limited Spanish and Indian resistance, a network of missions was destroyed; most of the population either was killed or captured, fled to larger Spanish and French outposts, or voluntarily joined the English.

The only major event of former Carolina Governor James Moore's expedition was the Battle of Ayubale, which marked the only large-scale resistance to the English raids. Significant numbers of the Apalachee, unhappy with the conditions they lived in under the Spanish, simply abandoned their towns and joined Moore's expedition. They were resettled near the Savannah and Ocmulgee Rivers, where conditions were only slightly better.

Moore's raiding expedition was preceded and followed by other raiding activity that was principally conducted by English-allied Creeks. The cumulative effect of these raids, conducted between 1702 and 1709, was to depopulate Spanish Florida beyond the immediate confines of Saint Augustine and Pensacola.