Eadweard Muybridge takes a series of photographs to prove that all four feet of a horse leave the ground when it runs; the study becomes the basis of motion pictures.

The Horse in Motion is a series of cabinet cards by Eadweard Muybridge, including six cards that each show a sequential series of six to twelve "automatic electro-photographs" depicting the movement of a horse. Muybridge shot the photographs in June 1878. An additional card reprinted the single image of the horse "Occident" trotting at high speed, which had previously been published by Muybridge in 1877.

The series became the first example of chronophotography, an early method to photographically record the passing of time, mainly used to document the different phases of locomotion for scientific study. It formed an important step in the development of motion pictures for years to come.

Muybridge's work was commissioned by Leland Stanford, the industrialist, former Governor of California and horseman, who was interested in horse gait analysis.

Eadweard Muybridge (; 9 April 1830 – 8 May 1904, born Edward James Muggeridge) was an English photographer known for his pioneering work in photographic studies of motion, and early work in motion-picture projection. He adopted the first name Eadweard as the original Anglo-Saxon form of Edward, and the surname Muybridge, believing it to be similarly archaic.Born in Kingston upon Thames in the United Kingdom, at age 20 he emigrated to America as a bookseller, first to New York City, and eventually to San Francisco. Planning a return trip to Europe in 1860, he suffered serious head injuries in a stagecoach crash in Texas. He spent the next few years recuperating in Kingston upon Thames, where he took up professional photography, learning the wet-plate collodion process, and secured at least two British patents for his inventions. He returned to San Francisco in 1867, a man with a markedly changed personality. In 1868, he exhibited large photographs of Yosemite Valley, and began selling popular stereographs of his work.

In 1874, Muybridge shot and killed Major Harry Larkyns, his wife's lover, but was acquitted in a controversial jury trial, on the grounds of justifiable homicide. In 1875, he travelled for more than a year in Central America on a photographic expedition.

Today, Muybridge is best known for his pioneering chronophotography of animal locomotion between 1877 and 1886, which used multiple cameras to capture the different positions in a stride, and for his zoopraxiscope, a device for projecting painted motion pictures from glass discs that pre-dated the flexible perforated film strip used in cinematography. From 1883–1886, he entered a very productive period at the University of Pennsylvania in Philadelphia, producing over 100,000 images of animals and humans in motion, occasionally capturing what the human eye could not distinguish as separate moments in time.

During his later years, Muybridge gave many public lectures and demonstrations of his photography and early motion picture sequences, returning frequently to England and Europe to publicise his work in cities such as London and Paris. He also edited and published compilations of his work, some of which are still published today, and which greatly influenced visual artists and the developing fields of scientific and industrial photography. He returned to his native England permanently in 1894. In 1904, the year of his death, the Kingston Museum was opened in his hometown, and it continues to house a substantial collection of his works in a dedicated gallery.