Dutch rebels led by Louis of Nassau, defeat Jean de Ligne, Duke of Arenberg, and his loyalist troops in the Battle of Heiligerlee, opening the Eighty Years' War.

The Battle of Heiligerlee (Heiligerlee, Groningen, 23 May 1568) was fought between Dutch rebels and the Spanish army of Friesland. It was the first Dutch victory during the Eighty Years' War.

The Groningen province of the Spanish Netherlands was invaded by an army consisting of 3,900 infantry, led by Louis of Nassau, and 200 cavalry, led by Adolf of Nassau. Both of them were brothers of William I of Orange. The intention was to begin an armed uprising against the Spanish rulers of the Netherlands.

The Stadtholder of Friesland and also Duke of Aremberg, Johan de Ligne, had an army of 3,200 infantry and 20 cavalry.

Aremberg initially avoided confrontation and awaited reinforcements from the Count of Meghem. However, on 23 May, Adolf's cavalry lured him to an ambush at the monastery of Heiligerlee. Louis's infantry made up the bulk of the army and defeated the Spanish force which lost 460 men, and the invading force lost 50, including Adolf. The rebels captured seven cannons.

The invading force, however, did not capture any cities and was soon defeated at the Battle of Jemmingen.

The death of Adolf of Nassau is mentioned in the Dutch national anthem (fourth verse):

Graef Adolff is ghebleven, In Vriesland in den slaech,

"Count Adolf stayed behind, in Friesland, in the battle"

Louis of Nassau (Dutch: Lodewijk van Nassau, January 10, 1538 – April 14, 1574) was the third son of William, Count of Nassau and Juliana of Stolberg, and the younger brother of Prince William of Orange Nassau.

Louis was a key figure in the revolt of the Netherlands against Spain and a strongly convinced Calvinist, unlike his brother William, whom he helped in various ways, including by arranging the marriage between him and his second wife Anna of Saxony. In 1569 William appointed him governor of the principality of Orange, giving him an indisputable position in French politics.