Queen Elizabeth I of England orders the arrest of Mary, Queen of Scots.

Mary, Queen of Scots (8 December 1542 8 February 1587), also known as Mary Stuart or Mary I of Scotland, was Queen of Scotland from 14 December 1542 until her forced abdication in 1567.

The only surviving legitimate child of James V of Scotland, Mary was six days old when her father died and she acceded to the throne. During her childhood, Scotland was governed by regents, first by the heir to the throne, James Hamilton, Earl of Arran, and then by her mother, Mary of Guise. In 1548, she was betrothed to Francis, the Dauphin of France, and was sent to be brought up in France, where she would be safe from invading English forces during the Rough Wooing. Mary married Francis in 1558, becoming queen consort of France from his accession in 1559 until his death in December 1560. Widowed, Mary returned to Scotland in August 1561. Following the Scottish Reformation, the tense religious and political climate that Mary encountered on her return to Scotland was further agitated by prominent Scots such as John Knox, who openly questioned whether her subjects had a duty to obey her. The early years of her personal rule were marked by pragmatism, tolerance, and moderation. She issued a proclamation accepting the religious settlement in Scotland as she had found it upon her return, retained advisers such as James Stewart, Earl of Moray, and William Maitland of Lethington, and governed as the Catholic monarch of a Protestant kingdom.

Mary married her half-cousin, Henry Stuart, Lord Darnley, in 1565, and in June 1566, they had a son, James. In February 1567, Darnley's residence was destroyed by an explosion, and he was found murdered in the garden. James Hepburn, 4th Earl of Bothwell, was generally believed to have orchestrated Darnley's death, but he was acquitted of the charge in April 1567, and the following month, he married Mary. Following an uprising against the couple, Mary was imprisoned in Loch Leven Castle. On 24 July 1567, she was forced to abdicate in favour of her one-year-old son. After an unsuccessful attempt to regain the throne, she fled southward seeking the protection of her first cousin once removed, Elizabeth I of England. (Elizabeth was the granddaughter of Henry VII of England, and Mary was his great-granddaughter.)

Mary had once claimed Elizabeth's throne as her own and was considered the legitimate sovereign of England by many English Catholics, including participants in a rebellion known as the Rising of the North. Perceiving Mary as a threat, Elizabeth had her confined in various castles and manor houses in the interior of England. After eighteen and a half years in captivity, Mary was found guilty of plotting to assassinate Elizabeth in 1586 and was beheaded the following year at Fotheringhay Castle. Mary's life, marriages, lineage, alleged involvement in plots against Elizabeth, and subsequent execution established her as a divisive and highly romanticised historical character, depicted in culture for centuries.

Elizabeth I (7 September 1533 – 24 March 1603) was Queen of England and Ireland from 17 November 1558 until her death in 1603. Sometimes referred to as the Virgin Queen, Elizabeth was the last of the five monarchs of the House of Tudor.Elizabeth was the daughter of Henry VIII and Anne Boleyn, his second wife, who was executed when Elizabeth was 21⁄2 years old. Anne's marriage to Henry was annulled, and Elizabeth was declared illegitimate. Her half-brother Edward VI ruled until his death in 1553, bequeathing the crown to Lady Jane Grey and ignoring the claims of his two half-sisters, the Roman Catholic Mary and the younger Elizabeth, in spite of statute law to the contrary. Edward's will was set aside and Mary became queen, deposing Lady Jane Grey. During Mary's reign, Elizabeth was imprisoned for nearly a year on suspicion of supporting Protestant rebels.

Upon her half-sister's death in 1558, Elizabeth succeeded to the throne and set out to rule by good counsel. She depended heavily on a group of trusted advisers led by William Cecil, whom much later she created 1st Baron Burghley. One of her first actions as queen was the establishment of an English Protestant church, of which she became the supreme governor. This Elizabethan Religious Settlement was to evolve into the Church of England. It was expected that Elizabeth would marry and produce an heir; however, despite numerous courtships, she never did. She was eventually succeeded by her first cousin twice removed, James VI of Scotland; this laid the foundation for the Kingdom of Great Britain. She had earlier been reluctantly responsible for the imprisonment and execution of James's mother, Mary, Queen of Scots.

In government, Elizabeth was more moderate than her father and half-siblings had been. One of her mottoes was "video et taceo" ("I see and keep silent"). In religion, she was relatively tolerant and avoided systematic persecution. After the pope declared her illegitimate in 1570 and released her subjects from obedience to her, several conspiracies threatened her life, all of which were defeated with the help of her ministers' secret service, run by Francis Walsingham. Elizabeth was cautious in foreign affairs, manoeuvring between the major powers of France and Spain. She only half-heartedly supported a number of ineffective, poorly resourced military campaigns in the Netherlands, France, and Ireland. By the mid-1580s, England could no longer avoid war with Spain.

As she grew older, Elizabeth became celebrated for her virginity. A cult of personality grew around her which was celebrated in the portraits, pageants, and literature of the day. Elizabeth's reign became known as the Elizabethan era. The period is famous for the flourishing of English drama, led by playwrights such as William Shakespeare and Christopher Marlowe, and for the prowess of English maritime adventurers such as Francis Drake and Walter Raleigh. Some historians depict Elizabeth as a short-tempered, sometimes indecisive ruler who enjoyed more than her fair share of luck. Towards the end of her reign, a series of economic and military problems weakened her popularity. However, Elizabeth is acknowledged as a charismatic performer and a dogged survivor in an era when government was ramshackle and limited, and when monarchs in neighbouring countries faced internal problems that jeopardised their thrones. After the short reigns of her half-siblings, her 44 years on the throne provided welcome stability for the kingdom and helped to forge a sense of national identity.