Nickelodeon Studios was a production studio and theme park attraction run by the television network Nickelodeon at Universal Studios Florida.

Opening on June 7, 1990, as The First World Headquarters for Kids, the studio attracted young tourists as contestants and audience members for Nickelodeon's live-action programming. At its peak, the studio employed 400 people and was the largest production studio in Florida, bringing $110 million in business to the state by 1994.

The studio closed permanently on April 30, 2005, after much of Nickelodeon's production had moved to Nickelodeon on Sunset. Nickelodeon Studios ended its run having produced over 2,000 episodes of original programming.