Antony and Cleopatra, Op. 40, is an opera in three acts by American composer Samuel Barber. The libretto was prepared by Franco Zeffirelli. It was based on the play Antony and Cleopatra by William Shakespeare and made use of Shakespeare's language exclusively.

The opera was first performed on September 16, 1966, commissioned for the opening of the new Metropolitan Opera House in Lincoln Center for the Performing Arts in New York City. After an unsuccessful premiere, the opera was extensively revised by Barber and Gian Carlo Menotti into an edition first performed in 1975.

The Metropolitan Opera House (also known as The Met) is an opera house located on Broadway at Lincoln Square on the Upper West Side of Manhattan in New York City. Part of Lincoln Center for the Performing Arts, the theater was designed by Wallace K. Harrison. It opened in 1966, replacing the original 1883 Metropolitan Opera House at Broadway and 39th Street. With a seating capacity of approximately 3,850, the house is the largest repertory opera house in the world. Home to the Metropolitan Opera Company, the facility also hosts the American Ballet Theatre in the summer months.